Journal: Cancer Informatics

Article Title: Exon-Specific Targeted Analysis of BRCA1 and BRCA2 Mutations in Bangladeshi Breast Cancer Patients

doi: 10.1177/11769351261445625

Figure Lengend Snippet: Validating the extraction of genomic DNA and PCR amplification of the desired size in 1.5% agarose gel: (A) agarose gel after genomic DNA extraction from blood samples. Genomic DNA of 5 samples is shown here in the first, D1-D5 lanes and 100 bp DNA ladder was loaded into the first lane (designated as M), (B) agarose gel after PCR amplification by the primers targeting BRCA1 exon 4 (A1) and 16 (A2) and BRCA2 exon 18 (A3), 23 (A4), and 25 (A5). Here, M denotes 100 bp DNA marker/ladder, (C) validating the presence of a specific PCR product after purification. In the first lane, 100 bp DNA ladder is denoted by M and the purified amplicons of BRCA1 exon 4 (P1) and 16 (P2) and BRCA2 exon 18 (P3), 23 (P4) and 25 (P5) are loaded from the second-sixth lanes, and (D) Agarose gel after PCR amplification by the BRCA2 F2R2 primer targeting exon 18 for patients S1 to S8.

Article Snippet: Genomic DNA from blood samples was extracted using FavorPrepTM Blood Genomic DNA Extraction Mini Kit and the genomic DNA was purified by using Monarch Genomic DNA Purification Kit (New England Biolabs), according to the manufacturer’s protocol.

Techniques: Extraction, Amplification, Agarose Gel Electrophoresis, DNA Extraction, Marker, Purification